Equipment and techniques
Available equipment and techniques
- Zeiss
Besides user-friendly, automatized and smart confocal microscopy, this microscope can be used to perform spectral detection (32 channels), fluorescence recovery after photobleaching (FRAP), fluorescence correlation spectroscopy (FCS, with or without the dynamic profiler). The inverted microscope is highly equipped with modern tools like the AI sample finder and automatic water immersion. Last but not least, the AiryScan detector can be used to perform super-resolution images, and/or to increase acquisition speed and sensitivity.
- Zeiss
This multimodal camera-based fluorescence microscope is our working horse for super-resolution microscopy. Using structured illumination microscopy (SIM), images at a resolution of typically 120 nm lateral resolution can be acquired at high frame rates, enabling live-cell imaging. Using single molecule localization microscopy methods like STORM, spatial resolution down to few 10s of nm can be reached. The microscope can also be used as a standard epifluorescence microscope with a total internal reflection (TIRF) option.
- Leica
Our second confocal microscope is also very versatile and extremely flexible. Thanks to the white light laser, lambda scans both in excitation and emission can be performed. Besides, it is equipped with all components to perform multiphoton microscopy as well as fluorescence lifetime microscopy.
- Zeiss
This epifluorescence microscope can accompany you for a wide range of projects requiring fast, accessible, user-friendly imaging. High throughput studies are possible thanks to the high-end automatization features, including the automatic refill of the water immersion.
Our powerful workstation can be booked like a microscope to perform demanding image processing tasks. It is equipped with a license for Imaris (4D visualization and a wide panel of image processing tasks), Huygens (deconvolution and more), LAS (processing of Leica data), and ZEN BLUE (processing of Zeiss data, including AiryScan processing)
For data management of microscopy images, we offer our users access to the OMERO server as well as support and workshops for the usage of OMERO.
All microscopes are located in a laboratory area at biosafety level 2 and possess the necessary incubation accessories for live cell imaging.
Further equipment and techniques
This lightsheet microscope has a particularly wide sample chamber, thus enabling extremely diverse applications, including on-chip imaging. Imaging of full volumes in relatively short acquisition times is thus possible.
These techniques enable unprecedented spatial resolution, which can be tuned from 50 nm down to below 10 nm. At least one of the STED microscopes is very user-friendly.
Inverted microscope, excitation wavelenghts: 355 nm (10mW), 457 nm (30mW), 532 nm (50mW), 785 nm (300mW), motorised control system, Okolab incubation chamber.
Upright microscope, excitation wavelength: 785 nm, automated cell recognition and acquisition of single cell spectra.
Chip platforms allow tailored sampling concepts for cell measurements. They combine a controllable microenvironment with optimal access to laser light focused by Raman microscopes or Raman probes.
These microscopes can be easily acquired.